doc. Ing. Petr Smýkal, Ph.D.

Information:

Education

• 1988 - 1994 graduated at Czech University of Agriculture,Prague and Faculty of Science, Charles University, Prague - interdisciplinary study of Plant Biotechnology
• 1994 - 1999 Department of Plant Physiology, Charles University, Prague

Professional experience

• 1993 - 94 Technical Assistant, Laboratory of plant tissue cultures, Czech University of Agriculture
• 1994 - 99 Research Assistant, Laboratory of Plant Molecular Biology, Dept. of Plant Physiology and Anatomy, Charles University, Prague
• 1999 - 2001 Research Assistant, Institute of Plant Science, Plant Genetics, Swiss Federal Institute of Technology, ETH ZURICH, Switzerland
• 2001- 2003, Research Assistant, Institute fur Biologie III, Faculty of Sciences, Albrecht Ludwig University of Freiburg, Germany
• 2004 - 2011, Research scientist at AGRITEC Plant Research Ltd. Department of Plant Biotechnology
• 2011 (untill now ) - Assistant Professor, Department of Botany, Faculty of Science, Palacký University, Olomouc

Fellowships

• 1992-93 JET TEMPUS, University of Cordoba, Spain, Microspore embryogenesis of cereals
• JET TEMPUS, Cambridge Science Park, Biogemma - Nickerson Seed Ltd, UK
• 1996 University of Nijmegen, Netherlands. Molecular and Cellular Aspects of Plant Reproduction
• 2005 University of Dundee at SCRI, A.Flavell laboratory
• 2006 OECD fellowship, A.Schulman, Genome Dynamics Laboratory, University of Helsinki
• 2008 Royal Society of Edinburgh fellowship, University of Dundee at SCRI
• 2012 Royal Society of Edinburgh fellowship, Royal Botanic Garden Ediburgh, fylogeneze Vicieae

Society Memberships

• European Federation of Plant Physiologist (FESPP)
• Czech Society of Experimental Plant Biology (ČSEBR)
• EUCARPIA
• International Legume Society (steering committee member)
• Czech Journal of Genetics and Plant Breeding (Editorial board member)
• Legume Perspectives (Editorial board member)
• Biologia Plantarum (Associate Editor)

Research Interest

My main focus now is the understanding of genetic changes made during the process of plant domestication, using wild pea as model to dissect genetic, chemical and biological principles governing two key domestication traits – seed dormancy and pod dehiscence. We use comparative analysis of wild and domesticated pea by wide range of genomics, transcriptomics and analytical chemistry approaches. Seed dormancy is also very important adaptive trait to environmental conditions, being studied by genome-wide association approaches and environmental factors using Medicago truncatula HapMap and wild Pisum sp. resources. Legumes in broader sense, with focus on Fabeae tribe, are of my interest in phylogeny too. Finally, crop wild relatives, again in pea context are being used for establishment of introgression lines in order to broaden genetic diversity of the crop which was lost as the consequence of domestication bottleneck.

Research Projects

• 1997-99: (Fysiologické a genetické mechanismy pylové embryogenese u rodu Brassica. COST- OC 822.60, MŠMT (odpovědný řešitel)
• 1996-98: Pylové embryogenese u Brassica napus. Grantová agentura Univezity Karlovy (odpovědný řešitel)
• 1996-98: Program "250" - "Posílení výzkumu na vysokých školách": Rostlinná buňka a stres. VS 96145. MŠMT (researcher)
• 1998-99: Regulace a signalizace v živých soustavách.Výzkumný záměr PřFUK, MSM 113100003 (researcher)
• 1999-2001: Transition on flowering in photoperiodic tobacco. National Science Foundation, Switzerland (researcher)
• 2001-2004: Role of Zwille in meristem paterning of Arabidopsis. DGA, Germany (researcher)
• 2003-2007: Tvorba transgenních linií hrachu (Pisum sativum L.) se zvýšenou odolností k virovým patogenům, QF3072, MZe (researcher)
• 2004: OC 843.70 Fyziologické a genetické aspekty somatické embryogeneze u hrachu (Pisum sativum L.). COST 843, MŠMT (researcher)
• 2008-2010: Technology Transfer Platform for breeding and growing of Grain legumes. EU program GL-TTP. INGO projekt MŠMT (principal researcher, coordinator)
• 2004-2010: Studium a využití biotechnologických a molekulárních metod v geneticko-šlechtitelském výzkumu rodu Pisum a Linum.) Výzkumný záměr MSM2678424601, MŠMT (researcher, administrator)
• 2006-2011: Semeno lnu pro zdraví člověka. 2B06087 NPV II projekt, MŠMT. (researcher)
• 2006-2011: Funkctional genomics and proteomics in plant breeding. Výzkumné centrum 1M06030. MŠMT. (researcher)
• 2009-2013: Konvenční a molekulárně-genetické přístupy při tvorbě luskovin rezistentních vůči virovým a houbovým chorobám a hmyzím škůdcům. QI91A229 MZe. (researcher)
• 2010-2012: Perfect markers for resistance genes in pea (Pisum sativum L.) to Fusarium root rot and Fusarium wilt. MŠMT, KONTAKT project with USDA-ARS, Pullman (principal researcher)
• 2010-2012: Construction of introgression lines carrying wild pea (Pisum fulvum/P. elatius) segments in cultivated pea (Pisum sativum L.): A tool for discovery of novel traits. MŠMT, KONTAKT project with Russian Academy of Sciences, Novosibirsk (principal researcher)
• 2010-2011: Towards comprehensive pea germplasm management for future use. LOA 10/048, AEGIS, Bioversity International, Rome. (principal researcher, coordinator)
• 2014-2017: FP7 LEGATO (LEGumes for the Agriculture of Tomorrow) n°FP7-613551, (principal researcher for UPOL, WP1 task 1.3 leader)
• 2014-2016: Seed dormancy and pod dehiscence as the key domestication traits in legumes. Grant Agency of Czech Republic (co-ordinator, principal researcher)
• 2016-2018: Ecological genomic approaches to uncovering the adaptive significance of seed dormancy in legumes. Grant Agency of Czech Republic (co-ordinator, principal researcher)

Teaching

• BOT/PVRSB: Plant Variation and Evolution
• KBB/KOGEN: Conservation Genetics
• BOT/OFGSB: Phytogenofond conservation
• BOT/MMSR: Molecular markers in plant breeding
• BOT/PRSB: Cultivated plants
• BOT/EABR: Ecology and applied biotechnology of plants
• BOT/SRO: Plant Breeding

Publications

• Renzi, J.P., Duchoslav, M. , Brus, J., Hradilová, I., Pechanec, V., Václavek, T., Machalová, J., Hron, K., Verdier, J., Smýkal, P. (2020): Physical dormancy release in Medicago truncatula seeds is related to environmental variations. - Plants 9(4), 503.[PDF]
• Renzi, J. P., Brus, J., Pirintsos, S., Erdős, L., Duchoslav, M. , Smýkal, P. (2020):Release of Medicago truncatula Gaertn. and Pisum sativum subsp. elatius (M. Bieb.) Asch. et Graebn. seed dormancy tested in soil conditions. - Agronomy 10: 1026.[PDF]
• Hradilová, I. , Duchoslav, M. , Brus, J., Pechanec, V., Hýbl, M., Kopecký, P., Smržová, L., Štefelová, N., Václavek, T., Bariotakis, M., Machalová, J., Hron, K., Pirinthos, S., Smýkal, P. (2019):Variation in wild pea (Pisum sativum subsp. elatius) seed dormancy and its relationship to the environment and seed coat traits. - PeerJ 6: e6263.[PDF]
• Nováková, E., Záblatzká, L., Brus, J., Majeský, Ľ. , Cvrčková, F., Kalendar, R., Smýkal, P. (2019):Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.). - International Journal of Molecular Sciences 20(7): 1773.
• Cechová, M., Hradilová, I. , Smýkal, P. , Barták, P., Bednář, P.(2019):Utilization of atmospheric solids analysis probe mass spectrometry for analysis of fatty acids on seed surface. - Analytical and Bioanalytical Chemistry 411: 1169–1180.[PDF]
• Janská, A., Pecková, E., Sczepaniak, B., Smýkal, P. , Soukup, A.(2019):The role of the testa during the establishment of physical dormancy in the pea seed. - Annals of Botany 123: 815–829.[PDF]
• Janská Anna, Pecková Eva, Sczepaniak Bogna, Smýkal, P. , Soukup Aleš(2018):The role of the testa during the establishment of physical dormancy in the pea seed. [PDF]
• Trněný Oldřich, Brus Jan, Hradilová, I. , Rathore Abhishek, Das Roma, Kopecký Pavel, Coyne Clarice, Reeves Patrick, Richards Christopher, Smýkal, P. (2018):Molecular evidence for two domestication events in the pea crop. [PDF]
• Smýkal, P. , Nelson Matthew, Berger Jens, von Wettberg Eric Bishop(2018):The Impact of Genetic Changes during Crop Domestication.[PDF]
• Cechová Monika, Hradilová, I. , Smýkal, P. , Barták Petr, Bednář Petr(2018):Utilization of atmospheric solids analysis probe mass spectrometry for analysis of fatty acids on seed surface. (in press)[PDF]
• Smýkal, P. , Trněný, O., Brus, J., Hanáček, P., Rathore, A., Roma, R.D., Pechanec, V., Duchoslav, M. , Bhattacharyya, D., Bariotakis, M., Pirintsos, S., Berger, J., Toker, C.(2018):Genetic structure of wild pea (Pisum sativum subsp. elatius) populations in the northern part of the Fertile Crescent reflects moderate cross-pollination and strong effect of geographic but not environmental distance. - Plos One 13(3): e0194056[PDF]
• Cechová Monika, Válková Markéta, Hradilová, I. , Soukup Aleš, Janská Anna, Smýkal, P. , Bednář Petr(2017):Towards Better Understanding of Pea Seed Dormancy using Laser Desorption/Ionization Mass Spectrometry[PDF]
• Smýkal, P. , Chaloupská, M., Bariotakis, M., Marečková, L. , Sinjushin, A., Gabrielyan, I., Akopian, J., Toker, C., Kenicer, G., Kitner, M. , Pirintsos, S.(2017): Spatial patterns and intraspecific diversity of the glacial relict legume species Vavilovia formosa (Stev.) Fed. in Eurasia. - Plant Systematics and Evolution 303(3): 267-282. DOI: 10.1007/s00606-016-1368-5 [PDF]
• Brus, J., Burian, T., Pechanec, V., Smýkal, P. , Duchoslav, M. (2017):Analysis of the local environmental conditions of legumes using global datasets. - 17th International Multidisciplinary Scientific GeoConference SGEM 2017, SGEM2017 Conference Proceedings, ISBN 978-619-7408-01-0 / ISSN 1314-2704, Vol. 17, Issue 21, 791-798 pp.[PDF]
• Tekin, M., Sari, D., Catal, M., Ikten, C., Smýkal, P. , Penmetsa, R.V., Von Wettberg, E.J., Toker, C.(2017):Eco-geographic distribution of Cicer isauricum P.H. Davis and threats to the species. - Genetic Resources and Crop Evolution 65: 67-77.[PDF]
• Hradilová, I. , Trněný, O., Válková, M., Cechová, M., Janská, A., Prokešová, L., Aamir, K., Krezdorn, N., Rotter, B., Winter, P., Varshney, R.V., Soukup, A., Bednář, P., Hanáček, P., Smýkal, P. (2017):A combined comparative transcriptomic, metabolomic, and anatomical analyses of two key domestication traits: Pod dehiscence and seed dormancy in pea (Pisum sp.). - Frontiers in Plant Sciences 8: 542.[PDF]
• Siol, M., Jacquin, F., Chabert-Martinello, M., Smýkal, P. , Le Paslier, M.-C., Aubert, G., Burstin, J.(2017):Patterns of Genetic Structure and Linkage Disequilibrium in a Large Collection of Pea Germplasm. - G3: Genes, Genomes, Genetics 7: 2461-2471.[PDF]
• Cechová, M., Válková, M., Hradilová, I. , Janská, A., Soukup, A., Smýkal, P. , Bednář, P.(2017):Towards Better Understanding of Pea Seed Dormancy using Laser Desorption/Ionization Mass Spectrometry. - International Journal of Molecular Sciences 18: 2196.[PDF]
• Smýkal, P. , Hradilová, I. , Trněný, O., Brus, J., Rathore, A., Bariotakis, M., Das, R. R., Bhattacharyya, D., Richards, C., Coyne, C. J., Pirintsos, S.(2017):Genomic diversity and macroecology of the crop wild relatives of domesticated pea. - Scientific Reports 7: 17384[PDF]
• Smýkal, P. , Varshney, R. K., Singh, V. K., Coyne, C. J., Domoney, C., Kejnovský, E., Warkentin, T.(2016):From Mendel’s discovery on pea to today’s plant genetics and breeding. - Theoretical and Applied Genetics.(in press)[PDF]
• Smýkal, P. , Coyne, C. J., Ambrose, M. J., Maxted, N., Schaefer, H., Blair, M. W., Berger, J., Greene, S. J., Nelson, M. W., Besharat, N., Vymyslický, T., Toker, C., Saxena, R. K., Roorkiwal, M., Pandey, M. K., Hu, J., Li, Y. H., Wang, L. X., Guo, Y., Qiu, L. J., Redden, R. J., Varshney, R. K.(2015):Legume crops phylogeny and genetic diversity for science and breeding. - Critical Reviews in Plant Sciences 34: 43–104.[PDF]
• Coyne, C. J., Pilet-Nayel, M. L., McGee, R. J., Porter, L. D., Smýkal, P. , Grünwald, N. J.(2015):Identification of QTL controlling high levels of partial resistance to Fusarium solani f. sp. pisi in pea. - Plant Breeding 134: 446–453.[PDF]
• Warkentin T., Smýkal, P. , Coyne C., Weeden N., Domoney C., Bing D., Leonforte T., Xuxiao Z., Dixit G., Boros L., McPhee K., McGee R., Burstin J., Ellis N.(2015):Pea. In: Grain Legumes, Handbook of Plant Breeding series. De Ron A. and Santalla M., Eds. Springer Science and Business Media.
• Konečná, E., Šafářová, D., Navrátil, M., Hanáček, P., Coyne, C., Flavell, A., Vishnyakova, M., Ambrose, M., Redden, R., Smýkal, P. (2014):Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm. - Plos One DOI: 10.1371/journal.pone.0090394[PDF]
• Smýkal, P. , Vernoud V., Blair MW., Soukup A., Thompson RD.(2014):The role of the testa during development and in establishment of dormancy of the legume seed. Front. Plant Sci. 5: 351. doi: 10.3389/fpls.2014.00351
• Mikič A., Smýkal, P. , and 21 co-authors(2014):Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. Planta 240 (5): 1139-1146.
• Smýkal, P. , and 8 coauthors(2014):A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. Genet Resour Crop Evol. 61:1533–1544
• Smýkal, P. (2014):Pea (Pisum sativum L.) in biology prior and after Mendel’s discovery. Czech. J. Genet. Plant Breed., 50: 52–64
• Schwarzbach E., Smýkal, P. , Dostál O., Jarkovská M., Valová S.(2014):Gregor J. Mendel – genetics founding father. Czech. J. Genet. Plant Breed., 50: 43–51.
• Warkentin, T., Smýkal, P. , Coyne, C., Weeden, N., Domoney, C., Bing, D., Leonforte, T., Xuxiao, Z., Dixit, G.(2014):Pea. In: Grain Legumes, Handbook of Plant Breeding series. De Ron A. and Santalla M., Eds. Springer Science and Business Media.
• Kwon, S. J., Smýkal, P. , Hu, J., Wang, M., Kim, S. J., McGee, R. J., McPhee, K., Coyne, C. J.(2013):User-friendly markers linked to Fusarium wilt race 1 resistance Fw gene for marker-assisted selection in pea. Plant Breeding 132, 642–648.
• Mikić, A., Smýkal, P. , Kenicer, G., Vishnyakova, M., Sarukhanyan, N., Akopian, J., Vanyan, A., Gabrielyan, I., Smýkalová, I., Sherbakova, E.(2013):The bicentenary of the research on 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential. Botanical Journal of the Linnean Society 172 (4): 524-531.
• Vrbová, M., Kotrba, P., Horacek, J., Smýkal, P. , Švábová, L., Větrovcová, M., Smýkalová, I., Griga, M.(2013):Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alpha-domain as a fusion to beta-glucuronidase protein. Plant Cell Tissue And Organ Culture 112(3): 321-330.
• De Beukelaer, H., Smýkal, P. , Davenport, G. F., Fack, V.(2013):Core Hunter II: fast core subset selection based on multiple genetic diversity measures using Mixed Replica search. BMC Bioinformatics 13: 312
• Smýkal, P. , Coyne, C., Redden, R., Maxted, N.(2013):Peas. In: Genetic and Genomic Resources of Grain Legume Improvement. Singh M. and Upadhyaya H., Eds. Elsevier, Netherlands.
• Smýkal, P. , G. Aubert, J. Burstin, C.J. Coyne, N.T.H. Ellis, A.J. Flavell, R. Ford, M. Hýbl, J. Macas, a kol.(2012):Pea (Pisum sativum L.) in the Genomic Era. Review. Open Access Agronomy 2: 74- 115
• Jing R., Ambrose M.A., Knox M.R., Smýkal, P. , Hybl M., Ramos Á., Caminero C., Burstin J., Duc G., a kol.(2012):Genetic Diversity in European Pisum Germplasm Collections. Theor Appl Genetics 125: 367-380
• Cieslarová J., Hýbl M., Griga M., Fialová E., Smýkal, P. (2012):Molecular Analysis of Temporal Genetic Structuring in Pea (Pisum sativum L.) Cultivars Bred in the Czech Republic and in Former Czechoslovakia Since the Mid-20th Century. Czech J Genet Pl Breed 48: 61-73.
• Smýkal, P. , Kenicer G., Flavell A.J., Corander J., Kosterin O., Redden R.J., Ford R., Coyne C.J., Maxted N., Ambrose M.J.(2011):Phylogeny, phylogeography and genetic diversity of the Pisum genus. Plant Genetic Resources 9: 4-18
• Smýkal, P. , Bačová-Kerteszová, N., Kalendar, R., Corander, J., Schulman, A.H., Pavelek, M.(2011):Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers. Theor Appl Genetics 122: 1385 - 1397
• Cieslarová, J., Smýkal, P. , Dočkalová, Z., Hanáček, P., Procházka, S., Hýbl, M., Griga, M.(2011):Molecular evidence of genetic diversity changes in pea (Pisum sativum L.) germplasm after long-term maintenance. Genet Resour Crop Evol. 58: 439-451
• Smýkal, P. (2011):Molekulární detekce genu rezistence hrachu k viru mozaiky přenosné semenem (PSbMV) 2011.
• Cieslarová J, Hanáček P., Fialová E., Hýbl M., Smýkal, P. (2011):Estimation of pea (Pisum sativum L.) microsatellite mutation rate based on pedigree and single-seed descent analyses. J Appl Genetics 52: 391-401
• Upadhyaya HD, Dwivedi SL, Ambrose M, Ellis N, Berger J, Smýkal, P. , D. Debouck D, Duc G, Dumet D, Flavell A(2011):Legume genetic resources: management, diversity assessment, and utilization in crop improvement. Euphytica 180: 27-47
• Cieslarová J., Hanáček P., Fialová E., Hýbl M., Smýkal, P. (2011):Estimation of pea (Pisum sativum L.) microsatellite mutation rate based on pedigree and single-seed descent analyses. J Appl Genetics 52: 391-401
• Zorić, L., Mikić, A., Akopian, J., Sarukhanyan, N., Gabrielyan, I., Vanyan, A., Luković, J., Smýkal, P. , Kenicer, G., Vishnyakova M. at all.(2010):Contributions to the characterization of Vavilovia formosa (syn. Pisum formosum.). I. Anatomy of stem, leaf and calyx. Pisum Genetics 42: 21-24
• Atlagić, J., Mikić, A., Sarukhanyan, N., Akopian, J., Vanyan, A., Gabrielyan, I., Smýkal, P. , Kenicer, G., Vishnyakova, M., Ambrose, M.(2010):Contributions to the characterization of Vavilovia formosa (syn. Pisum formosum). II. Morphology of androecium and gynoecium and mitosis. Pisum Genetics 42: 25-27
• Zeremski-Škorić, T., Mikić, A., Sarukhanyan, N., Akopian, J., Vanyan, A., Gabrielyan, I., Smýkal, P. , Kenicer, G., Vishnyakova, M., Ambrose, M.(2010):Contributions to the characterization of Vavilovia formosa (syn. Pisum formosum). III. Contents of macro- and microelements. Pisum Genetics 42: 28-30
• Kalendar, R., Antonius, K., Smýkal, P. , Schulman, A.(2010):A universal method for DNA fingerprinting and retrotransposon isolation. Theor Appl Genetics. 121:1419-30.
• Jing, R., Vershinin, A., Grzebyta, J., Shaw, P., Smýkal, P. , Marshall, D., Ambrose, M.J., Ellis,T.H.N., Flavell, A.J.(2010):The genetic diversity and evolution of field pea (Pisum) studied by high throughput retrotransposon based insertion polymorphism (RBIP) marker analysis. BMC Evolutionary Biology 10: 44.
• Mikič, A., Smýkal, P. , Kenicer, G., Sarukhanyan, N., Akopian, J., Gabrielyan, I., Vanyan, A., Sinjushin, A., Demidenko, N., Čupina, B. at all.(2010):Achievements in Research on Vavilovia (Vavilovia formosa (Stev.) Fed.), a Legume Crop Wild Relative. Field Veg. Crop Res. 47: 387-394.
• Smýkal, P. , Šafářová, D., Navrátil, M., Dostalová, R.(2010):Marker assisted pea breeding: eIF4E allele specific markers to pea seed-borne mosaic virus (PSbMV) resistance. Mol Breeding, 26: 425-438.
• Smýkal, P. (2010):Genetika a genomika v luskovinách. In: Houba - Hochman - Hosnedl a kol. (ed.) LUSKOVINY, pěstování a uţití. (ISBN 978-80-87111-19-2) 2010, p. 59-67 .
• Akopian J., Sarukhanyan N., Gabrielyan I., Vanyan A., Mikić A., Smýkal, P. , Kenicer G., Vishnyakova M., Sinjushin A., Demidenko N.(2010):Reports on establishing an ex situ site for 'beautiful' vavilovia (Vavilovia formosa) in Armenia. Genet Resour Crop Evol. 57: 1127–1134
• Horáček, J., Griga, M., Smýkal, P. , Hýbl, M.(2009):Effect of Environmental and Genetic Factors on the Stability of Pea (Pisum sativum L.) Isozyme and DNA Markers. Czech J. Genet. Plant Breed. 45: 57–71.
• Smýkal, P. , Kalendar, R., Ford, R., Macas, J., Griga, M.(2009):Evolutionary conserved lineage of Angela-like retrotransposons as a genome-wide microsatellite repeat dispersal agent. Heredity 103: 157–167.
• Mikič, A., Smýkal, P. , Kenicer, G., Vishnyakova, M., Sarukhanyan, N., Akopian, J., Gabrielyan, I., Vanyan, A., Čupina, B., Mihailovič V. at all.(2009):A revival of research on beautiful vavilovia (Vavilovia formosa syn. Pisum formosanum). Pisum Genetics 41: 11-14.
• Vrbová, M., Horáček, J., Smýkal, P. , Griga, M.(2009):Flax (Linum usitatissimum L.) transformation with heavy metal binding protein genes. In: Sehnal F., Drobník J. (Eds.) White Book Of Genetically Modified Crops. EU regulations and research experience from the Czech Republic. Biology Centre AS CR České Budějovice (ISBN 978-80-86668-05-3) 2009, p. 57.
• Švábová, L., Griga, M., Navrátil, M., Šafářová, D., Hanáček, P., Reinöhl, V., Horáček, J., Smýkal, P. (2009):Transgenic pea (Pisum sativum L.) lines with improved tolerance to Pea Enation Mosaic Virus and Pea Seed-borne Mosaic Virus. In: Sehnal F., Drobník J. (Eds.) White Book Of Genetically Modified Crops. EU regulations and research experience from the Czech Republic. Biology Centre AS CR České Budějovic
• Smýkal, P. , Hýbl, M., Corander, J., Jarkovský, J., Flavell, A., Griga, M.(2008):Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theor Appl Genetics 117: 413-424.
• Smýkal, P. , Coyne, C.J., Ford, R., Redden, R., Flavell, A.J., Hýbl, M., Warkentin, T., Burstin, J., Duc, G., Ambrose, M. at all.(2008):Effort towards a world pea (Pisum sativum L.) germplasm core collection: The case for common markers and data compatibility. Pisum Genetics 40: 11-14.
• Smýkal, P. , Horáček, J., Dostálová, R., Hýbl, M.(2008):Variety discrimination in pea (Pisum sativum L.) by molecular, biochemical and morphological markers. J Appl Genetic 49: 155-166.
• Švábová, L., Smýkal, P. , Griga, M.(2008):Agrobacterium-mediated transformation of pea (Pisum sativum L.): Transformant production in vitro and by non-tissue culture approach. In: M. C. Kharkwal (ed.), Food Legumes for Nutritional Security and Sustainable Agriculture, New Delhi, India, p.208-220 (ISBN 978-81-908995-2-9).
• Smýkal, P. , Valledor, L., Rodríguez, R., Griga, M.(2007):Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.). Plant Cell Rep. 26: 1985-98.
• Smýkal, P. , Gennen, J., De Bodt, S., Ranganath, V., Melzer, S.(2007):Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches.Plant Mol Biol. 65: 233-42.
• Smýkal, P. (2006):Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification. J Appl Genetic 47: 221–230.
• Švábová, L., Smýkal, P. , Griga, M., Ondřej, V. (2005):Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo. Biol. Plant. 49(3), 361-370.
• Švábová, L., Smýkal, P. , Griga, M., Ondřej, V. (2005):Agrobacterium-mediated transformation of Pisum sativum by in vitro and in vivo approach. Biologia Plantarum 49: 361-370.
• Smýkal, P. , Gleissner, R., Corbesier, L., Apel, K., Melzer, S.(2004):Modulation of flowering responses in different Nicotiana varieties. Plant Molecular Biology 55: 253-262.
• Pechan, P.M., Smýkal, P. (2001):Androgenesis: affecting the fate of the male gametophyte. Physiologia Plantarum, 111, 1- 8.
• Fidlerová, A., Smýkal, P. , Tupý, J., Čapková, V.(2001):Glycoproteins 66 and 69 kDa of pollen tube wall: properties and distribution in angiosperms. J.Pl.Physiology 158: 1367-1374.
• Chandler, J., Smýkal, P. , Melzer, S.(2001):FPF1 and flowering time control via GA signalling. Flowering Newsletter 31: 19-24.
• Smýkal, P. (2001):Pollen embryogenesis - the stress mediated switch from gametophytic to sporophytic development. Current status and future prospects. Review. Biologia Plantarum 43, 481-489.
• Chandler, J., Smýkal, P. , Melzer, S.(2001):Recent Research Developments in Plant Physiology, Vol. 2 In.: S.G.Pandalai (ed.), The transition to flowering, (ISBN:81-7736-046-9) 2001, p. 259-269.
• Smýkal, P. , Pechan, P.M.(2000):Stress, as assessed by the appearance of sHsp transcripts, is required but not sufficient to initiate androgenesis. Physiologia Plantarum 110, 135-144.
• Smýkal, P. , Mašín, J., Hrdý, I., Konopásek, I., Ţárský, V.(2000):Chaperone activity of tobacco HSP18, a small heat-shock protein, is inhibited by ATP. Plant J. 23, 703-713.
• Rosel, D., Puta, F., Blahušková, A., Smýkal, P. , Folk, P.(2000):Molecular characterization of a calmodulin-like Dictyostelium protein CalB. FEBS Lett. 473, 323-327.
• Smýkal, P. , Hrdý, I., Pechan, P.M.(2000):High-molecular-mass complexes formed in vivo contain smHSPs and HSP70 and display chaperone-like activity. Eur J Biochem. 267, 2195-207.
• Smýkal, P. , Janotová, I., Pechan, P.M.(2000):A novel Brassica napus L. pollen-specific gene belongs to a nucleic-acid-binding protein family. Sex Plant Reprod. . 13, 127-134, 2000.
• Mašek, T., Smýkal, P. , Janotová, I., Honys,D., Čapková,V., Pechan, P.(2000):Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group MHG I/Y protein. Plant Science 159, 197 – 204.